Acute leukemia cells injected in NSG mice generated faster and more efficient leukemic characteristics compared with other NOD- scid-related strains. Similarly, higher tumorigenicity in NSG mice has been reported with neuroblastoma 1 and melanoma 15 cells. NOG mice have shown superiority in cancer xenoplantation systems compared with nude, scid, and NOD- scid mice, when human cervical cancer, 12 pancreatic cancer, 13 and multiple myeloma 14 cell lines were used. Cg- Prkdc scid Il2rg tm1Sug/Jic mice (abbreviated as NOD/Shi- scid Il2rg −/− and often referred to as NOG mice), C.Cg- Rag2 tm1Fwa Il2rg tm1Sug mice (abbreviated as BALB/c- Rag2 −/− Il2rg −/− mice) and Stock (H2 d)- Rag2 tm1Fwa Il2rg tm1Krf mice (referred to as H2 d- Rag2 −/− Il2rg −/− mice).Cg- Prkdc scid Il2rg tm1Wjl/SzJ mice (abbreviated as NOD/LtSz- scid Il2rg −/− and often referred to as NSG mice), NOD.9– 11 The immunodeficient strains of Il2rg −/− mice include NOD 8, 9 Absence of the IL-2R γ-chain in mice leads to severe impairments in T- and B-cell development and function, and completely prevents natural killer (NK) cell development. The IL-2R γ-chain is a crucial component of the high-affinity receptors for IL-2, IL-4, IL-7, IL-9, and IL-15, and is required for signaling through these receptors. The interleukin-2 receptor (IL-2R) γ-chain is known as the common cytokine receptor γ-chain. 2 To date, the most extensively used immunodeficient mouse strains for developing lung cancer xenograft models include nude, 3 scid, 4 and non-obese diabetic (NOD)- scid 5– 7 mice. 1 Immunodeficient mice have also been important for investigating carcinogenesis, cancer therapy, and imaging of tumor growth and metastasis. Immunodeficient mice that cannot reject xenotransplanted cells have been shown to be the best living recipients for developing xenograft models of human cancer. To overcome these restrictions, mouse models have been generated. The study of cancer in humans is impeded by several reasons, such as inaccessibility to the tumor sites, difficulty in the assessment of tumor biology, and ethical concerns. Keywords: NOD- scid Il2rg(null), tumorigenicity, xenotransplantation, xenograft, mouse model We confirmed that the engrafted tumors originated from inoculated human lung cancer cells by immunohistochemical staining with human cytokeratin and vimentin.Ĭonclusion: NSG mice may be the most suitable strain for testing tumorigenicity of lung cancer, especially if only a few cells are available. Tumor formation was observed only in NSG mice after inoculation of 10 3 or fewer A549 cells (40% vs 0% in 15 NSG mice compared with others, respectively, P=0.0169). NSG mice similarly showed higher susceptibility to xenotransplantation of A549 cells. When 10 3 EBC1 cells were injected, no tumors developed in any strain other than NSG mice, while tumorigenesis was achieved in all the five NSG mice (100%, P=0.0079) within 9 weeks. Tumors developed in two of the five NOD- scid mice (40%) and in all the five NSG mice (100%). Results: When 10 4 EBC1 cells were inoculated, no tumor formation was observed in BALB/ c-nu or C.B-17 scid mice. Various numbers (10 1–10 5 cells/head) of two lung cancer cell lines, A549 and EBC1, were subcutaneously inoculated and tumor sizes were measured every week up to 12 weeks. Materials and methods: We directly compared the susceptibility of four immunodeficient mouse strains, c-nu, C.B-17 scid, NOD- scid, and NOD/LtSz- scid Il2rg-/- (NSG) mice, for tumor formation from xenotransplanted lung cancer cell lines. The purpose of this study is to select a suitable mouse strain as a xenogenic host for testing tumorigenicity of lung cancer. Purpose: No lung cancer xenograft model using non-obese diabetic (NOD)- scid Il2rg-/- mice has been reported. Nobuhiro Kanaji, 1 Akira Tadokoro, 1 Kentaro Susaki, 1 Saki Yokokura, 1 Kiyomi Ohmichi, 2 Reiji Haba, 2 Naoki Watanabe, 1 Shuji Bandoh, 1 Tomoya Ishii, 1 Hiroaki Dobashi, 1 Takuya Matsunaga 1ġDepartment of Internal Medicine, Division of Hematology, Rheumatology and Respiratory Medicine, Faculty of Medicine, Kagawa University, Kagawa, Japan 2Department of Diagnostic Pathology, Faculty of Medicine, Kagawa University, Kagawa, Japan
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